Abstract
This research was conducted to evaluate the effect of turmeric water extract, garlic and combination turmeric and garlic as a feed additive in the broiler diet on pancreatic enzyme activity of broiler chicken. Effectivity of treatments was assessed by addition of phytobiotic (control, 015% zinc bacitracin, 2.5% TE, 2.0% GE, 2.5% TGE) which were arranged Completely Randomized Design with 4 replications. The variables measured were pancreatic enzyme activity(amylase enzyme activity, protease enzyme activity and lipase enzyme activity).The results showed that enzyme protein activity content of 2.5% TE supplementation is also high at 82.02 U/ml, then supplemented 2.5% TGE, 2.0% GE, negative control and positive control respectively 75.98 ; 72.02; 68.74; and 66.57 U/ml. The lipase enzyme activity whereas the negative control and a positive control differ significantly higher (P<0.05) to treatment with the addition of 2.5% TE, 2.0% GE and 2.5% TGE phytobiotic. The research concluded that the incorporation of 2.5% TE, 2% GE and combined 2.5% TGE as feed additive enhanced pancreatic enzyme activity.
Full Text:
PDFReferences
Darusman, L.K., Rohaeti E., Sulistiyani, 2001. Kajiansenyawagolongan flavonoid asaltanaman bangle sebagai senyawa peluruh lemak melalui aktivitas lipase. Pusat Studi Biofarmaka Lembaga Penelitian, IPB, Bogor.
Engberg, R.M., Hedeman M.S., Leser T.D., Jensen B.B., 2000. Effect of Zinc Bacitracin and Salinomycin on intestinal microflora and performance of broilers. Poult. Sci. 79: 1311 1319.
Esimone, C.O., Okoye, F.B.C. Odomegwu, D.C., Nworu, C.S.,Oleghe, P.P,Ejogha, P.W., 2010. In vitro antimicrobial evaluation of lozenges containing extract of garlic and ginger. Int. J. of Health Res. 3 (2): 105 110.
Kwan, K.K.H., Nakai, S.,Skura, B.J., 1983. Comparison of four methods for determining protease activity in milk. J. Food Sci. 48 (5): 1418 1421.
Linfield, W.M., O’Brien, D.J.,Serota, S., Barauskas, R.A., 1984. Lipid-lipase interactions. I. Fat splitting with lipase from Candida rugosa. J. Am. Oil Chem Soc.61: 1067 1071.
Lopez, P., Sanchez, C., Batlle, R., Nerin, C., 2005. Solid-and vapor-phase antimicrobial activities of six essential oils: Susceptibility of selected foodborne bacterial and fungal strains. J. Agr. Food Chem. 53: 6939 6946.
Hammer, K.A., Carson, C.F., Riley, T.V., 1999. Antimicrobial activity of essential oils and other plant extracts. J. App. Microbiol. 86: 985 990.
Han, L.K., Kimura, Y., Kawashima, M., Takaku, T.,Taniyama, T., Hayashi, T., Zheng, Y-N.,Okuda, H., 2001. Anti-obesity effects in rodents of dietary teasaponin, a lipase inhibitor. Int. J. Obesity. 25: 1459 1464.
Han, L.K., Zheng, N.Y., Yoshikawa, M., Okuda, H., Kimura, Y., 2005. Anti-obesity effects of chikusetsusaponins isolated from Panaxjaponicusrhizomes. BioMed Central. 5: 1 10.
Han, L.K., Nose, R., Li, W., Gong, X.J., Zheng, Y.N., Yoshikawa, M., Koike, K., Nikaido, T., Okuda, H., Kimura, Y., 2006. Reduction of fat storage in mice fed a high-fat diet long term by treatment with saponins prepared from Kochiascoparia fruit. Phythoter. Res. 20: 877 882.
Hashemi, S.R., Davoodi, H., 2010. Phytogenics as new class of feed additive in poultry industry. J. Anim. Vet. Adv. 9 (17): 2295 2304.
Hung, W.C. Chen, F.Y., Lee, C.C., Sun, Y., Huang, H.W., 2008. Membrane-thinning effect of curcumin. J. Biophys. 94: 4331 4338.
Jamroz, D., Wiliczkiewicz, A., Wertelecki, T., Orda, J., Skorupinska, J., 2005. Use of active substances of plant origin in chicken diets base om maize and locally grown cereals. Brit. Poult. Sci. 46: 485 493.
Jang, I.S., Ko, Y.H., Yang, H.Y., Ha, J.S., Kim, J.Y., Kang, S.Y.,Yoo, D.H., Nam, D.S., Kim, D.H., Lee, C.Y., 2004. Influence of essensial oil components on growth performances and the functional activity of the pancreas and small intestine in broiler chickens. Asian-australas. J. Anim. Sci. 17: 394 400.
Joshita, D., Azizahwati, Wahyuditomo, 2000. Pengaruhdaunjatibelandaterhadapkerjaenzim lipase secarain vitro. Warta TumbuhanObat Indonesia 6 (2): 16 22.
Khan, R.U., Naz, S.,Javdani, M.,Nikousefat, Z., Selvaggi, M., Tufarelli, V., Laudadio, V., 2012. The use of Turmeric (Curcuma longa) in poultry feed. World's Poult. Sci. J.68: 97 103.
Kolev, T. M., 2005. DFT and experimental studies of the structure and vibrational spectra of curcumin. International Journal of Quantum Chemistry. 102 (6): 1069 1079.
Madigan, M., 2005. Brock Biology of Microorganisms. London. Prentice Hall.
Maiorka, A., da Silva, A.V.F., Santin, E., 2004. Broiler breeder age and dietary energy level on performance and pancreas lipase and trypsin activities of 7-days old chicks. Int. J. Poult. Sci. 3 (3): 234 237.
Malathi, S., Chakraborty, R., 1991. Production of alkaline protease by a New Aspergillusflavus isolate under solid substrate fermentation conditions for use as a depilation agent. Appl. Environ. Microbiol. 57 (3): 712 716.
Marczylo, T.H., Verschoyle, R.D., Cooke, D.N., Morazzoni, P., Steward, W.P., Gescher, A.J., 2006. Comparison of systemic availability of curcumin with that of curcumin formulated with phosphatidylcholine . Cancer ChemotherPharmacol. 60: 171 177.
Merkley, J.W., Weinland, B.T., Malone, G.W., Chaloupka, G.W., 1980. Evaluation of five commercial broiler crosses. 2. Eviscerated yield and component parts. Poult. Sci., 59: 1755-1760.
National Research Council (NRC), 1994. Nutrient Requirements of Poultry. Ed Rev ke-9. Washington DC: Academy Pr.
Panda, A., Rao, S.R., Raju, M., 2006. Natural growth promoters have potential in poultry feeding systems. Feed tech. 10 (8): 23 35.
Panda, A.,Rao, S.R., Raju, M., 2009. Phytobiotics, a natural growth promoter. Poultry International. pp: 10 11.
Platel, K., Srinivasan, K., 2004. Digestive stimulant action of spices: A myth or reality? The Indian J. Med. Res.119: 167 179.
Platel, K.,Srinivasan, K., 2001. Studies on the influence of dietary spices on food transit time in experimental rats. Nutr. Res. 21: 1309 1314.
Platel, K., Srinivasan, K., 2000. Influence of dietary spices and their active principles on pancreatic digestive enzymes in albino rats. Food/Nahrung. 44: 42 46.
Ramakrishna, R., Platel, K., Srinivasan, K., 2003. In vitro influence of spices and spice-active principles on digestive enzymes of rat pancreas small intestine. Nahrung/Food. 47: 408 412.
Routman, K.S., Yoshida, L.,Frizzas de Lima, A.C., Maccari, M.,Pizauro, Jr. J.M., 2003. Intestinal and pancreas enzyme activity of broilers exposed to thermal stress. Braz. J. Poult. Sci. 5 (1): 23 27.
Shin, J.E, Han, M.J.,Kim, D.H., 2003. 3-Methylethergalangin isolated from Alpiniaofficinarum inhibits pancreatic lipase. Biol. Pharm Bull. 26 (6): 854 857.
Soeka, Sudaryati, Y., Djajasukma, E., 1993. Pengaruhpenambahansumber-sumber nitrogen terhadapaktivitasenzim alpha amilaseAspergillusniferdalam media campuranonggokdandedak. Prosiding Seminar HasilLitbang SDH.
Steel, R.G.D., Torrie, J.H., 1995. Prinsip dan Prosedur Statistika. Suatu Pendekatan Biometrik. Ed ke-2. Ir. Bambang Sumantri, penerjemah. GM: Penerbit PT Gramedia Pustaka Utama. Terjemahan dari : Principles and Procedures of Statistics.
Suarni, Patong, R., 2007. Potency of Mung bean sprout as enzyme source (-amilase). Indo. J. Chem. 7 (3): 332 336.
Xu, B.J., Han, L.K., Zheng, Y.N., Lee, J.H.,Sung, C.K., 2005. In vitro inhibitory effect of triterpenoidalsaponins from Platycodi radix on pancreatic lipase. Arch. Pharm. Res. 28 (2): 180 185.
Williams, P., Losa, R., 2001. The use of essential oils and their compound in poultry nutrition. World’s Poult. Sci. J. 17: 14 15.
DOI: http://dx.doi.org/10.20884/1.jap.2015.17.3.518
Article Metrics
##”article.abstract”## view : 1323 timesPDF – 801 times
Article Metrics
Metrics powered by PLOS ALM
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.