Iman Sukirman, Eros Sukmawati, Siti Darojah Rasad, Nurcholidah Solihati

Abstract


This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.

Keywords


breed; diluents type; motility; intact plasma membrane

Full Text:

PDF

References


Aboagla, E. M. E. and T. Terada. 2004. Effects of Egg Yolk during the Freezing Step of Cryopreservation on the Viability of Goat Spermatozoa. Theriogenology. 62(2004):1160–1172.

Anwar, P., Y. S. Ondho and D. Samsudewa. 2015. Kualitas Membran Plasma Utuh Dan Tudung Akrosom Utuh. Agromedia. 33(1):53-63.

Ariantie, O. S., T. L. Yusuf, D. Sajuthi and R. I. Arifiantini. 2013. Effect of Glycerol and Dimethylformamide Cryoprotectants on Buck Etawah Crossbreed Frozen Semen Using Modified Tris Diluents. Jurnal Ilmu Ternakdan Veteriner. 18(4):239-250.

Arifiantini, I. and T. L. Yusuf. 2004. Keberhasilan Penggunaan Tiga Pengencer Dalam Dua Jenis Kemasan pada Proses Pembekuan Semen Sapi Frisien Holstein. Fakultas Kedokteran Hewan, Institut Pertanian Bogor.

Arifiantini, I. and T. L. Yusuf. 2010. Developing of Tris Soy Milk Diluent for Frisian Holstein Bull Frozen Semen. HAYATI Journal of Biosciences. 17(2):91-94.

Arifiantini, I., T. L. Yusuf and N. Graha. 2005. Longivitas dan Recovery Rate Pasca Thawing Semen Beku Sapi Fresian Holstein Menggunakan Bahan Pengencer Berbeda. Buletin Peternakan. 29(2): 53-61.

Ax, R. L., M. Dally, B. A. Didion, R. W. Lenz, C. C. Love, D. D. Varner, B. Hafez and M. E. Bellin. 2008. Semen Evaluation. B Hafez, ESE Hafez, Reproduction in Farm Animals. 7th edition. Lippincott Williams and Wilkins, Philadelphia. 365-368, 378.

Baharun, A., I. Arifiantini and T. L. Yusuf. 2017. Freezing Capability of Pasundan Bull Sperm Using Tris-Egg Yolk, Tris-Soy, and Andromed® Diluents. Jurnal Kedokteran Hewan. 11(1):45-49.

Cooter, P. Z., H. A. Goolsby, S. D. Prien. 2005. Preliminary Evaluation of a Unique Freezing Technology for Bovine Sperm Cryopreservation. Reprod Dom Animal. 40:98-99.

Gaspersz, V. 1995.Teknik Analisis dalam Penelitian Percobaan. Penerbit Tarsito. Bandung.

Ghareeb, S., W. Haron, R. Yusoff, N. Yimer, F. Baiee, T. Ahmedeltayeb and M. Ebrahimi. 2017. Post-Thaw Evaluation of Cryopreserved Bull Semen Extended In Four Different Semen Extenders. Australian Journal of Basic and Applied Sciences. 11(5):80-87.

Gunawan, M., F. Afiati, E. M. Kaiin, S. Said and B. Tappa. 2004. Pengaruh media Pengencer Terhadap Kualitas Spermatozoa Beku Sapi PO. Seminar Nasional Teknologi Peternakan dan Veteriner. 61-66.

Kusumawati, E. D. and H. Leondro. 2011. Kualitas Semen Segar Sapi Pejantan Pada Penyimpanan dan Lama Simpan yang Berbeda. Jurnal Veteriner. 15(1):433-439.

Moce, E. and J. K. Graham. 2006. Cholesterol-Loaded Cyclodextrins Added to Fresh Bull Ejaculatesimprove Sperm Cryosurvival. J. Anim. Sci. 84:826–833.

Mostari, M. P., M.G.M. Rahman, M.A.M.Y. Khandoker and S.S. Husain. 2004. Evaluation of Bulls Based on Semen Quality and Herd Fertility. Pakistan Journal of Biological Scinces. 7(12):2177-2181.

Moussa, M., V. Martinez, A. Trimeche, D. Tanturier and M. Anton. 2002. Low Density Lipoprotein Extracted From Hen Egg Yolk By an Easy Method: Cryoprotective effect on Frozen-Thawed Bull Semen. Theriogenology. 57:1591-1762.

Parrish, J. 2003. Techniques in Domestic Animal Reproduction - Evaluation and Freezing of Semenhttp://www.wisc.edu/ansci_repro/(accesed: January25 2018).

Paulenz, H., L. Soderquist, R. P. Perez and K. A. Berg. 2002. Effect of Different Extender and Storage Temperatures on Sperm Viability of Liquid Ram Semen. Theriogenology. 57:823-836.

Permatasari, W. D., E. T. Setiatin, and D.

Samsudewa. 2013. Studi Tentang Pengencer Kuning Telur dan Pengaruhnya Terhadap Kualitas Semen Beku Sapi Jawa Brebes. Animal Agriculture Journal. 2(1):143 – 151.

Priyanto, L., R. I. Arifiantini and T. L. Yusuf. 2015. Deteksi Kerusakan DNA Spermatozoa Semen Segar dan Semen Beku Sapi Menggunakan Pewarnaan Toluidine Blue. Jurnal Veteriner. 16(1):48-55.

Rahmawati, M. A., T. Susilawati and M. N. Ihsan. 2015. Kualitas Semen dan Produksi Semen Beku pada Bangsa Sapi dan Bulan Penampungan yang Berbeda. Jurnal Ilmu-Ilmu Peternakan 25 (3): 25 – 36.

Rezki, Z. M., D. Samsudewa and Y. S. Ondho. 2016. Pengaruh Pengencer Kombinasi Sari Kedelai dan Tris terhadap Kualitas Mikroskopis Spermatozoa Pejantan Sapi PO Kebumen. Jurnal Sain Peternakan Indonesia. 11(2):67-74.

Solihati, N., R. Idi, S. D. Rasad, M. Rizal, and M. Fitriati. 2008. Kualitas Sperma Cauda Epididimis Sapi Peranakan Ongole (PO) dalam Pengencer Susu, Tris dan Sitrat Kuning Telur pada Penyimpanan 4-5oC. Animal Production. 10(1):22-29.

Sukmawati, E. 2014. Daya Tahan Spermatozoa terhadap Proses Pembekuan pada Berbagai Jenis Sapi Pejantan Unggul. Tesis. Sekolah Pascasarjana. Institut Pertanian Bogor.

Wahyuningsih, A., D. M. Saleh and Sugiyatno. 2013. Pengaruh Umur Pejantan dan Frekuensi Penampungan Terhadap Volume dan Motilitas Semen Segar Sapi Simmental di Balai Inseminasi Buatan lembang. Jurnal Ilmiah Peternakan. 1(3): 947-953.

Watson, P. F. 2000. The Causes of Reduced Fertility with Cryopreserved Semen. Animal Reproduction Science 60–61 _2000. 481–492.

Widjaya, N. 2011. Pengaruh Pemberian Susu Skim dengan Pengencer Tris Kuning Telur Terhadap Daya Tahan Hidup Spermatozoa Sapi pada Suhu Penyimpanan 5⁰C. Sains Peternakan. 9(2):72-76.

Zega, I., S. Ilyas and S. Hutahaean. 2015. Kualitas Spermatozoa Sapi Limousin Dalam Pengencer Two-Stetm Extender Dengan Suplementasi Kuning Telur Bebek Selama Penyimpanan Pada Refrigerator. Jurnal Biosains. 1(3):66-72.




DOI: http://dx.doi.org/10.20884/1.jap.2019.21.2.641

Article Metrics

##”article.abstract”## view : 272 times
PDF – 76 times

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Powered by OJS | Design by ThemeOJS