The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG

Abstract

Twenty cattles were used in this experiment to determine the  quantity and  quality of embryo after injected FSH (follicle stimulating hormone ) and  PMSG (pregnant mare serum gonadotrophin) in Brahman Cross Cattle. The experiment was assigned into Completely Randomized Design with 5 treatments and 4 replications. The treatments were T₁ = 4 mg of FSH twice a day intra-ovary decreased doses, T₂ = 8 mg of FSH twice a day intra-ovary decreased doses, T3 = 300 IU of PMSG single dose intra-ovary, T₄ = 600 IU of PMSG single dose intra-ovary, T₅ = 40 mg of FSH twice a day intramuscular decreased doses. Trial cattle were oestrus synchronized using 15 mg of PGF2α that gave twice at 11-daily intervals. One day after giving FSH and PMSG was detected the cattle’s oestrus. Washing uterus  was done at day 7 after AI using mixture of PBS, FCS and streptomicyn. Data observed were cow performances, embryo quantity and embryo quality. Results of experiment showed that 19 cattle (95%) responded oestrus synchronized treatment and super ovulation, whereas 1 cattle (5%) did not respond oestrus synchronized treatment and super ovulation. Generally, cattle showed oestrus at 2 – 3 days after giving PGF2α. Eleven cattle (57,90%)  showed oestrus at 2 days after giving PGF2α whereas the others (8 cattle, 42,10%) showed oestrus 3 days giving PGF2α. The treatment of giving FSH and PMSG could increase (P<0,05) embryo. T₅ was highest  compared the others ( T₁, T₂, T₃ and T₄), while T₂ and T₄ were higher than T₁ and T₃. Produced total embryo was 82 with average 4,3 ± 5,67 using FSH and PMSG. 8 embryo (9,76%), 9 embryos (10,90), 20  embryo (24,40%), 16 embryo (19,50%) and  29 embryos (35,40%) were grade A, B, C, D and E respectively. It is concluded that giving of 40 mg  FSH  intramusculer produce the best embryo donor whereas  and giving  of FSH 8 mg intraovari was the best effeciency. (Animal Production 11(2): 96-102 (2009)

Key Words : Brahman Cross Cattle, embryo, PGF₂α PMSG, FSH